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NEW YORK, NEW YORK - SEPTEMBER 01: Serena Williams and Venus Williams of The United States look on during a changeover against Lucie Hradecka and Linda Noskova of Czech Republic during the Women’s Doubles First Round match on Day Four of the 2022 US Open at USTA Billie Jean King National Tennis Center on September 01, 2022 in the Flushing neighborhood of the Queens borough of New York City. (Photo by Elsa/Getty Images)

Venus and Serena Williams have played their final doubles match together.  The superstar sisters lost in the first round of the US Open doubles match Thursday night.

Arthur Ashe stadium had never hosted a first-round doubles match for women or men until this match.  This was the first time the sisters had partnered up since the 2018 French Open.  Combined, they have 14 Grand Slam titles in doubles.

Serena Williams’ historic U.S. Open run will continue on Friday, September 2nd and it could be the last time that we see her on the court.  The 23-time Grand Slam winner has said she plans to retire after the U.S. Open.  After wins in the first two rounds, Williams will face Ajla Tomljanović in Round 3 on Friday, airing on ESPN2.

Check out pictures of the sisters below.